Proteolytic Activity in Soybean Root Nodules

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Proteolytic Activity in Soybean Root Nodules : Activity in Host Cell Cytosol and Bacteroids throughout Physiological Development and Senescence.

Root nodules were harvested from chamber-grown soybean (Glycine max L. Merrill cv Woodworth) plants throughout development. Apparent nitrogenase activity (acetylene reduction) peaked before seeds began to develop, but a significant amount of activity remained as the seeds matured. Nodule senescence was defined as the period in which residual nitrogenase activity was lost. During this time, solu...

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Proteolytic Activity in Soybean Root Nodules 1 ACTIVITY IN HOST CELL CYTOSOL AND BACTEROIDS THROUGHOUT PHYSIOLOGICAL DEVELOPMENT AND SENESCENCE

Root nodules were harvested from chamber-grown soybean (Glycine max L. Merril cv Woodworth) plants throughout development. Apparent nitrogenase activity (acetylene reduction) peaked before seeds began to develop, but a significant amount of activity remained as the seeds matured. Nodule senescence was defined as the period in which residual nitrogenase activity was lost. During this time, solub...

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Peptidohydrolases of Soybean Root Nodules

Nodule extracts prepared from Glycine max var Woodworth possessed endopeptidase, aminoptidase, and carboxypeptidase actiities. Three distinct endopeptidase activities could be resolved by disc-gel electrophoresis at pH 8.8. According to their order of increasing electrophoretic mobility, the first of these enzymes hydrolyzed azocasein and n-benzoyl-LLeu-p8-naphthylamide, while the second hydrol...

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Ascorbate peroxidase from soybean root nodules.

AP is involved in the destruction of harmful H202. In soybean (Glycine m a x [LI Merr.) root nodules, AP initiates a sequence of coupled redox reactions (ascorbate-GSH pathway) that results in peroxide scavenging (Dalton et al., 1986). The ascorbate-GSH pathway occurs in other plant tissues and has been extensively studied in chloroplasts where photoreducing conditions lead to the production of...

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Glutathione S-transferases (GSTs) are ubiquitous enzymes that catalyze the conjugation of toxic xenobiotics and oxidatively produced compounds to reduced glutathione, which facilitates their metabolism, sequestration, or removal. We report here that soybean (Glycine max) root nodules contain at least 14 forms of GST, with GST9 being most prevalent, as measured by both real-time reverse transcri...

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ژورنال

عنوان ژورنال: Plant Physiology

سال: 1983

ISSN: 0032-0889,1532-2548

DOI: 10.1104/pp.71.4.797